Dornase alfa ( proprietary name Pulmozyme from Genentech ) is a highly purified solution of recombinant human deoxyribonuclease I ( rhDNase ), an enzyme which selectively cleaves DNA.

Dornase alfa is the most recent therapeutic agent developed with this basic mechanism of action.

Dornase alfa is an orphan drug.

Dornase alfa hydrolyzes the DNA present in sputum / mucus of cystic fibrosis patients and reduces viscosity in the lungs, promoting improved clearance of secretions.

In solution 75 mL ( 1mg / mL ) of Dornase Alfa typically costs $ 2150 USD.

Dornase alfa has recently been shown to improve lung function in non-cystic fibrosis pre-term infants suffering from atelectasis where other therapies have failed.

Purified DNA ligase is used in gene cloning to join DNA molecules together to form recombinant DNA.

This is called genetic engineering and was made possible through the discovery of DNA and the creation of the first recombinant DNA molecules by Paul Berg in 1972.

An organism that is generated through the introduction of recombinant DNA is considered to be a genetically modified organism.

The Lanthanum cation La < sup > 3 +</ sup > is a positive allosteric modulator at native and recombinant GABA receptors, increasing open channel time and decreasing desensitization in a subunit configuration dependent manner.

Lemna is being used as a recombinant expression system for economical production of complex biopharmaceuticals.

E. coli is the most widely studied prokaryotic model organism, and an important species in the fields of biotechnology and microbiology, where it has served as the host organism for the majority of work with recombinant DNA.

A recombinant uricase enzyme ( rasburicase ) is available ; its use, however, is limited, as it triggers an autoimmune response.

Chromosomal crossover ( or crossing over ) is the exchange of genetic material between homologous chromosomes that results in recombinant chromosomes.

A recombinant immunoblot assay is used to verify the immunoassay and the viral load is determined by a HCV RNA polymerase chain reaction.

While protein-mediated transduction supports reprogramming adult cells to pluripotency, the method is cumbersome and requires recombinant protein expression and purification expertise, and reprograms albeit at very low frequencies ( Kim et al., 2009 ).

In genetic engineering, recombination can also refer to artificial and deliberate recombination of disparate pieces of DNA, often from different organisms, creating what is called recombinant DNA.

This is followed by selection and screening of recombinant clones.

Somatotropin ( STH ) refers to the growth hormone 1 produced naturally in animals, whereas the term somatropin refers to growth hormone produced by recombinant DNA technology, and is abbreviated " HGH " in humans.

Andrew is responsible for recreating an extinct species, the Ragna Demon, through recombinant DNA experiments.

A genetically modified organism ( GMO ) is an organism whose genetic material has been altered using the genetic engineering techniques generally known as recombinant DNA technology.

Since the structure of a glycan may depend on the expression, activity and accessibility of the different biosynthetic enzymes, it is not possible to use recombinant DNA technology in order to produce large quantities of glycans for structural and functional studies as it is for proteins.

Erythropoietin is available as a therapeutic agent produced by recombinant DNA technology in mammalian cell culture.

This interest in recombinant ecosystems or ' weedscapes ' is partly inspired by a 1979 visit to New Zealand and interactions with New Zealand ecologist Haikai Tane ( 1995 ).

There is no ideal therapy for neutropenia, but recombinant G-CSF ( granulocyte-colony stimulating factor ) such as filgrastim can be effective in chemotherapy patients, in patients with congenital forms of neutropenia including severe congenital neutropenia, autosomal recessive Kostmann's syndrome, cyclic neutropenia, and myelokathexis.

The thrombin cleavage site ( Leu-Val-Pro-Arg-Gly-Ser ) is commonly included in linker regions of recombinant fusion protein constructs.

It is the gait of the human who must run to live: arms dangling, legs barely swinging over the ground, head hung down and only occasionally swinging up to see the target, a loose motion that is just short of stumbling and yet is wonderfully graceful.

To my knowledge, Lincoln remains the only Head of State and Commander-in-Chief who, while fighting a fearful war whose issue was in doubt, proved man enough to say this publicly -- to give his foe the benefit of the fact that in all human truth there is some error, and in all our error, some truth.

Even in domains where detailed and predictive understanding is still lacking, but where some explanations are possible, as with lightning and weather and earthquakes, the appropriate kind of human action has been more adequately indicated.

Though sex in some form or other enters into all human activity and it was a good thing that Freud emphasized this aspect of human nature, it is fantastic to explain everything in terms of sex.

As Lipton puts it: `` The Eros is felt in the magic circle of marijuana with far greater force, as a unifying principle in human relationships, than at any other time except, perhaps, in the mutual metaphysical orgasms.

But Aristotle kept the principle of levels and even augmented it by describing in the Poetics what kinds of character and action must be imitated if the play is to be a vehicle of serious and important human truths.

Whitehead contends that the human way of understanding existence as a unity of interlocking and interdependent processes which constitute each other and which cause each other to be and not to be is possible only because the basic form of such an understanding, for all its vagueness and tendency to mistake the detail, is initially given in the way man feels the world.

Individual human strength is needed to pit against an inhuman condition.

What is simply an opinion formed in defiance of the laws of human probability, whether or not it is later confirmed, has become by September of the election year `` a firm conviction ''.

Thus human perception and human volition is the immanent cause of all social change and this most truly when the change reaches the civilizational level.

As a Humanist, Dr. Huxley interests himself in the possibilities of human development, and one thing we can say about this suggestion, which comes from a leading zoologist, is that, so far as he is concerned, the scientific outlook places no rigid limitation upon the idea of future human evolution.

This text from Dr. Huxley is sometimes used by enthusiasts to indicate that they have the permission of the scientists to press the case for a wonderful unfoldment of psychic powers in human beings.

It seems quite obvious that all the really difficult tasks of human beings arise from the fact that man is not one, but many.

He is a utopian with a stake in tomorrow and he is a vulnerable human made captive by the circumstances of today.

There is another kind of ardor, a quiet, sure devotion to the fundamental decencies of human life, but no angry utopian contentions.