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Once considered a technical snag in cell fractionation techniques, the alleged ER vesicle contaminants that invariably appeared in the mitochondrial fraction have been re-identified as membranous structures derived from the MAM — the interface between mitochondria and the ER.
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Some Related Sentences
Once and considered
Once the toads were established, a 1963 study concluded, as the toad's diet included both harmful and beneficial invertebrates, it was considered " economically neutral ".
Once the usefulness of digitalis in regulating the human pulse was understood, it was employed for a variety of purposes, including the treatment of epilepsy and other seizure disorders, which are now considered to be inappropriate treatments.
Once a play is over, and before the next play starts, the football is considered dead.
Once completed, the depiction of Christ and the Virgin Mary naked was considered sacrilegious, and Cardinal Carafa and Monsignor Sernini ( Mantua's ambassador ) campaigned to have the fresco removed or censored, but the Pope resisted.
Once considered a passing fad, twin-tip skis have become a staple in the product line of all major ski-producing companies worldwide, with a few specializing in twin tips.
Once Colossus was built and installed, it ran continuously, powered by dual redundant diesel generators, the wartime mains supply being considered too unreliable.
Historian John Brooks in his book Once in Golconda considered the turn of the 19th to 20th century period to have been Wall Street's heyday.
Once recognized as discoverers, the team at GSI considered the names darmstadtium ( Ds ) and wixhausium ( Wi ) for element 110.
Once the fair idea was conceived, several sites were considered.
Once it has considered subsequences of length 1, it goes on to subsequences of length 2, and so on.
Once considered a primary skill, increased accessibility to more user-friendly DTP software has made DTP a secondary skill to art direction, graphic design, multimedia development, marketing communications, administrative careers and advanced high school literacy in thriving economies.
Once buffalo were located, hunting did not begin until the medicine men of the tribe considered the time propitious.
Once considered the world's fastest man by virtue of his multiple world records in the 60-yard, 100-yard, 220-yard, and Olympic 100-meter dashes, Hayes is the only man to win both an Olympic gold medal and a Super Bowl ring.
Once considered as an obsolete technique from the early days of photography, pinhole photography is from time to time a trend in artistic photography.
Once a show has opened ( premiered before a regular audience ), theatre directors are generally considered to have fulfilled their function.
Once, a member ( Georges de Porto-Riche ) was not accorded a reception because the eulogy he made of his predecessor was not considered satisfactory, and he refused to rewrite it.
Once again the harvesting activity was halted while the College took stock of its forest resources and considered its future management options.
Now considered politically incorrect, the borough had a wooden sign posted downtown that read " Once there was Indians all over this place " which had been donated by a resident who insisted on the wording of the sign as having been a quotation from an author.
Once considered the second most important settlement in New Mexico, the town is now masked by its popular tourist neighbors Taos and Santa Fe.
Once it is installed it has become “ attached to the land ” ( the house ) and is now considered real property.
Once considered primarily a red light district, the area is now much more mixed, with bars, pubs, restaurants and discos.
Once brain death has been declared the person can be considered for organ donation.
Once considered for the role of James Bond, Lucan was a charismatic man with expensive tastes ; he raced power boats and drove an Aston Martin.
Once considered the " Rose Capital of the World ", Richmond Hill has in recent years seen a huge population upsurge, being Canada's fastest-growing community in the 1990s.
Once and technical
Once we have made clear that we are genuinely concerned with a country's development potential, we can be blunt in suggesting the technical conditions that must be met for development to occur.
Once this complex is formed, it is identified via either a " tag " attached directly to the antibody, or added in an additional technical step.
Once the technical competition had expanded from the challenges of cracking to the challenges of creating visually stunning intros, the foundations for a new subculture known as demoscene were established.
Once this breakthrough had been achieved, detailed technical data was rapidly extracted by the use of this tool.
Once oxygen has been eliminated, perhaps the second most important technical factor in working below 200 nm is to design the rest of the optical system to have low losses in this region.
Once all arable land was under cultivation, the lack of technical progress meant that crop yields were relatively flat, whereas the population continued to grow.
Once founded, the regional technical college grew very quickly as a result of the obviously strong regional need for tertiary education.
Once programmers are familiar with PROIV then, consistent with the nature of PROIV as a 4GL, productivity in PROIV's intended application domains is typically high and programmers can be effective even without extensive technical skills / knowledge.
Once the hyperreal number system ( an infinitesimal-enriched continuum ) is in place, one has successfully incorporated a large part of the technical difficulties at the foundational level.
Once more Barnard was able to name his terms and opened a new technical office in Surrey named Ferrari Design and Development ( FDD ).
Once again a number of problems dirigenciales and constant technical changes not allowed the team to fulfill a good season.
Once that occurs, a technical foul is charged if a player remains in the game after his sixth or subsequent foul, or as the last player to foul out, re-enters the game in case of injury to an eligible player that must be removed.
Once established in this new position, Giorgini resumed his artistic work, combining his technical expertise with computers from his engineering training with his background in the visual arts, thereby becoming one of the first computer artists.
Once and cell
Once the virus has infected the cell, it will start replicating itself, using the mechanisms of the infected host cell.
Once inside the cell, the disulfide bond is reduced, and the A1 subunit is freed to bind with a human partner protein called ADP-ribosylation factor 6 ( Arf6 ).
Once inside, the DNA becomes expressed by the cell machinery, resulting in the production of therapeutic protein, which in turn treats the patient's disease.
Once inside the cell, they bind to and activate estrogen receptors which in turn modulate the expression of many genes.
Once activated, p53 will induce a cell cycle arrest to allow either repair and survival of the cell or apoptosis to discard the damaged cell.
Once the cell receives a physiological, pathological, or pharmacological stimulus, a number of cellular proteins are modified biochemically by signal cascades.
Once in the host's cell, the RNA strands undergo reverse transcription in the cytoplasm and are integrated into the host's genome, at which point the retroviral DNA is referred to as a provirus.
Once inside the periplasm, the Bdellovibrio cell seals the membrane hole and converts the host cell to a spheroblast.
Once this fourth division has occurred the embryo has reached a 16 cell stage.
Once colonization has begun, the biofilm grows through a combination of cell division and recruitment.
Once an embolism is formed, it usually cannot be removed ( but see later ); the affected cell cannot pull water up, and is rendered useless.
Once the elders returned to the cell, the seal was still in place, but Joseph was gone.
Once in the synapse, dopamine binds to and activates postsynaptic dopamine receptors, resulting in the signal of the presynaptic cell being propagated to the postsynaptic neuron.
Once a virus genome becomes operational in a host cell, it then generates messenger RNA ( mRNA ) molecules that direct the synthesis of viral proteins.
Once a tyrosine receptor kinase is bonded to its ligand, it is able to bind to tyrosine kinase residing in the cytosol of the cell.
Once the PMCs disrupt all attachment to their former location, the cells themselves change their morphology by contracting their apical surfaces, apical constriction, and enlarging their basal surfaces, thus acquiring a “ bottle cell ” phenotype.
Once the unit cell of a new phase is known, the next step is to establish the stoichiometry of the phase.
Once in the plasma, ribavirin is transported through the cell membrane also by nucleoside transporters.
Once this has happened, the row is " open " ( the desired cell data is available ).
The chromosomes ’ connection allows the cells to join together — making sure that the two copies are “ lined up together at the center of the dividing cell .” Once the chromosomes are aligned, tiny molecular spindles attach to each copy and then pull away the “ original and new copy ” as the division continues.
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