Once the door was open, they crowded him inside the dark building.

Once inside the luxuriosly-upholstered landau, she drew the curtains and proceeded to give the startled youth the kind of physical examination usually reserved for army inductees.

Once inside, keep your eyes open ''!!

Once inside his apartment, he poured a drink with trembling hands and flopped limply in a chair.

Once inside the fortification, Alfred realised, the Danes enjoyed the advantage, better situated to outlast their opponents or crush them with a counter attack as the provisions and stamina of the besieging forces waned.

Once inside, they pressurized the LM cabin, went through a half-hour briefing with scientists in mission control, and configured the cabin for the sleep period.

Once the tip penetrates the animal the upper sub head broke off from the rest of the shaft, however, since it was still connected with the braided loop it rotated the head into a horizontal position inside the animal ’ s body so that it could not get away from the hunter.

Once inside the host, the trypomastigotes invade cells, where they differentiate into intracellular amastigotes.

Once inside the cell, the disulfide bond is reduced, and the A1 subunit is freed to bind with a human partner protein called ADP-ribosylation factor 6 ( Arf6 ).

Once this post-translational modification process has been completed, the protein begins to fold ( sometimes spontaneously and sometimes with enzymatic assistance ), curling up on itself so that hydrophobic elements of the protein are buried deep inside the structure and hydrophilic elements end up on the outside.

Once inside the embassy, diplomatic channels can be used to solve the issue and send the refugees to another country.

Once inside the lumen, the molecules are modified, then sorted for transport to their next destinations.

Once inside, the DNA becomes expressed by the cell machinery, resulting in the production of therapeutic protein, which in turn treats the patient's disease.

Once the case is sized down, the inside of the neck of the case will actually be slightly smaller than the bullet diameter.

Once the punch has been evaded, the boxer " weaves " back to an upright position, emerging on either the outside or inside of the opponent's still-extended arm.

Once inside the lumen of the lymphatic vessels, the fluid is guided along increasingly larger vessels, first with rudimentary valves to prevent backflow, which later develop into complete valves similar to the venous valve.

Once inside, they used stolen keys to enter two apartments being used by the Israeli team at 31 Connollystraße.

Once inside the cell, they bind to and activate estrogen receptors which in turn modulate the expression of many genes.

Once inside, Blazkowicz destroys the V-2 on its launchpad and fights his way out of the facility towards an airbase filled with experimental jet aircraft.

Once inside, they called for backup from the Sixth Precinct using the bar's pay telephone.

Once clear, the two sides of the skin are distinct: the side facing inside the animal and the hair side.

Once inside the skin, dengue virus binds to Langerhans cells ( a population of dendritic cells in the skin that identifies pathogens ).

Once inside the host, the larvae live inside the haemocoel and absorb nutrients directly through their skin.

Once the virus has infected the cell, it will start replicating itself, using the mechanisms of the infected host cell.

Once considered a technical snag in cell fractionation techniques, the alleged ER vesicle contaminants that invariably appeared in the mitochondrial fraction have been re-identified as membranous structures derived from the MAM — the interface between mitochondria and the ER.

Once activated, p53 will induce a cell cycle arrest to allow either repair and survival of the cell or apoptosis to discard the damaged cell.

Once the cell receives a physiological, pathological, or pharmacological stimulus, a number of cellular proteins are modified biochemically by signal cascades.

Once in the host's cell, the RNA strands undergo reverse transcription in the cytoplasm and are integrated into the host's genome, at which point the retroviral DNA is referred to as a provirus.

Once this fourth division has occurred the embryo has reached a 16 cell stage.

Once colonization has begun, the biofilm grows through a combination of cell division and recruitment.

Once an embolism is formed, it usually cannot be removed ( but see later ); the affected cell cannot pull water up, and is rendered useless.

Once the elders returned to the cell, the seal was still in place, but Joseph was gone.

Once in the synapse, dopamine binds to and activates postsynaptic dopamine receptors, resulting in the signal of the presynaptic cell being propagated to the postsynaptic neuron.

Once a virus genome becomes operational in a host cell, it then generates messenger RNA ( mRNA ) molecules that direct the synthesis of viral proteins.

Once a tyrosine receptor kinase is bonded to its ligand, it is able to bind to tyrosine kinase residing in the cytosol of the cell.

Once the PMCs disrupt all attachment to their former location, the cells themselves change their morphology by contracting their apical surfaces, apical constriction, and enlarging their basal surfaces, thus acquiring a “ bottle cell ” phenotype.

Once the unit cell of a new phase is known, the next step is to establish the stoichiometry of the phase.

Once in the plasma, ribavirin is transported through the cell membrane also by nucleoside transporters.

Once this has happened, the row is " open " ( the desired cell data is available ).

The chromosomes ’ connection allows the cells to join together — making sure that the two copies are “ lined up together at the center of the dividing cell .” Once the chromosomes are aligned, tiny molecular spindles attach to each copy and then pull away the “ original and new copy ” as the division continues.

Once the tank made it to shore, all covers and seals could be blown off via explosive cables, enabling normal combat operation.

Once born ( usually within 9 months ' time ) the foramen ovale seals over.

Once home to a large population of elephant seals, hunters greatly reduced the seal population by overhunting the species.

Once all the Nightwatch members were gathered in the docking area, Zack Allan seals them in between airlocks before narrowly escaping himself, revealing that he had turned on Nightwatch, allied with Sheridan, and had just led Nightwatch into a trap.

Once heavier than about 250 g, the fish no longer become prey for birds and many fish, although seals do prey upon them.

Once all his seals are removed, he can unleash the full power of all the souls he has devoured, effectively summoning them under his control.