After dialysis the sample was centrifuged and the supernatant placed on a Af cm column of EEAE-cellulose equilibrated with starting buffer.

After starting his writing career by writing for " true confession " style pulp magazines like True Story, van Vogt decided to switch to writing something he enjoyed, science fiction.

After leaving school at 17, Ayckbourn's career took several temporary jobs in various places before starting a temporary job at the Scarborough Library Theatre, where he was introduced to the artistic director, Stephen Joseph.

After the tumultuous and disappointing 2007 season, the Falcons made a number of moves, hiring a new General Manager and head coach, drafting a new starting quarterback, and signing a starting running back.

After rallying his troops near Schwennenbach – well beyond their starting point – Eugene prepared to launch a second attack, led by the second-line squadrons under the Duke of Württemberg-Teck.

After the victory Boston's owner sold its star pitcher, Babe Ruth, to the New York Yankees to help funding of a Broadway play, and thus starting a tale of futility which would last 86 years, known as Curse of the Bambino.

After starting the season 15 – 35, though, the team had some success later in the year, going a respectable 30 – 28 in August and September as the youngsters became more experienced.

After defeating, but not subjugating, the Kingdom of Scotland, he declared himself rightful heir to the French throne in 1338, starting what would be known as the Hundred Years ' War.

After passing his examinations in 1752, he soon progressed through the merchant navy ranks, starting with his promotion in that year to mate aboard the collier brig Friendship.

After various Korean border conflicts, war broke out with U. S .- allied South Korea in 1950, starting the Korean War.

After cutting out the hatchlings may lie in their eggshells for hours before starting to dig out of the nest.

After being sent on a lengthy tour of Europe starting 1927 – including London, Paris and towns in Germany – during which he demonstrated his invention to full audiences, Theremin found his way to the United States, arriving on 30 December 1927 with his first wife Katia.

After the 1997 death of Scott Krueger, a new member at the Phi Gamma Delta fraternity, MIT required all freshmen to live in the dormitory system starting in 2002.

After starting a collaboration on heart applications with Robert Weiss at Johns Hopkins, Bottomley returned to the university in 1994, as Russell Morgan Professor and Director of the MR Research Division.

After the introduction of the motel, auto camps continued in popularity through the Depression years and after World War II, their popularity finally starting to diminish with the construction of freeways and changes in consumer demands.

After starting 3 – 7 in 1994, the Giants won their final six games to finish 9 – 7 but missed the playoffs.

After starting 5 – 0 in the 2009 season, New York lost to the likewise undefeated New Orleans Saints at the Superdome 48 – 27, beginning a four game losing streak, in which they lost to the Arizona Cardinals 24 – 17, the San Diego Chargers 21 – 20 and the Philadelphia Eagles 40 – 17.

After La Russa retired as manager of the Cardinals in 2011, Smith became active in the organization again, starting with his stint as a special instructor for the team's 2012 spring training camp.

After everyone has made their decision, the replacement cards are dealt starting at the dealer's left as usual.

After reaggravating a hamstring injury during Game 3 of the 2007 ALDS, Joe Torre removed Roger Clemens from the team's starting rotation.

After starting the engine with a known setting that allowed it to idle, the air valve was opened until maximum engine speed was obtained.

After a while, Prokofiev's mother felt that the isolation in Sontsovka was restricting his further musical development, yet his parents hesitated over starting their son on a musical career at such an early age.

After the Welsh victory at the battle of Llwchwr in January 1136 and the successful ambush of Richard Fitz Gilbert de Clare in April, south Wales rose in rebellion, starting in east Glamorgan and rapidly spreading across the rest of south Wales during 1137.

After 1890 came philosopher Josiah Royce ( 1855 – 1916 ), botanist Liberty Hyde Bailey ( 1858 – 1954 ), the Southern Agrarians of the 1920s and 1930s, novelist John Steinbeck ( 1902 – 1968 ), historian A. Whitney Griswold ( 1906 – 1963 ), environmentalist Aldo Leopold ( 1887 – 1948 ), Ralph Borsodi ( 1886 – 1977 ), and present-day authors Wendell Berry ( b. 1934 ), Gene Logsdon ( b. 1932 ), Paul Thompson, and Allan C. Carlson ( b. 1949 ).

After a protracted development cycle the initial AAM-N-2 Sparrow entered limited operational service in 1954 with specially modified Skyknights all weather carrier night fighters.

After 52 often beleaguered years in St. Louis, the Browns moved to Baltimore in 1954 and adopted the Orioles name in honor of the official state bird of Maryland.

After defeating the Boston Red Sox in the Division Series and the Seattle Mariners in the ALCS, Cleveland clinched a World Series berth, for the first time since 1954.

After filming Lucky Me with Phil Silvers and Young at Heart ( both 1954 ) with Frank Sinatra, Day chose not to renew her contract with Warner Brothers.

After the immigration station closed in November 1954, the buildings fell into disrepair and were all but abandoned.

After World War II, Taglioni designed engines for Ceccato motorcycles and Mondial before joining Ducati in 1954.

After playing with the Indianapolis Clowns of the Negro American League and in the minor leagues, Aaron started his major league career in 1954.

After a short period of Democratic Party administration, Yoshida returned in late 1948 and continued to serve as prime minister until 1954.

After several reorganizations of the armed forces, in 1954 the Japan Self-Defense Forces were established under a civilian director.

After hosting the annual event since 1954, he was to continue serving as the association's national chairman.

After obtaining his Ph D, he was a research fellow at Trinity College, Cambridge and then from 1954 a lecturer in the mathematics faculty at Cambridge.

After the war, between 1945 – 1954, the total annual catch increased and reached a maximum of 4, 900 tonnes.

After centuries of isolation, two devastating regional wars, and a civil war, in 1954 Paraguay entered a period of prolonged political and economic stability under the authoritarian rule of Alfredo Stroessner Mattiauda.

After spending months perfecting the recipe, Mr. Marrero created and sold the first piña colada on August 15, 1954, while working as the head bartender at the Caribe Hilton International Hotel, the most popular hotel in Puerto Rico among the 1950s.

After divorcing Rissone in France in 1954, he married Mercader in 1959, again in Mexico, but this union was not considered valid under Italian law.

After their defeat at Dien Bien Phu in 1954 France granted independence to the nations of Cambodia, Laos and Vietnam.

After transferring to Purdue University in the fall of 1954, Chaffee earned a bachelor of science degree in aeronautical engineering in 1957.

After World War II, a small number of technically able sopranos, the most notable of whom were first Maria Callas ( with performances from 1952 and especially those at La Scala and Berlin in 1954 / 55 under Herbert von Karajan ) and then Dame Joan Sutherland ( with her 1959 performances at the Royal Opera House Covent Garden in 1959, which were repeated in 1960 ), revived the opera in all of its original tragic glory.

After playing the lead role in Two Nights with Cleopatra ( 1953 ), her breakthrough role was in The Gold of Naples ( 1954 ), directed by Vittorio De Sica.

After the publication of The Lord of the Rings ( 1954 – 1955 ), the grammar rules of his major Elvish languages Quenya, Telerin and Sindarin went through very few changes ( this is late Elvish 1954 – 1973 ).

After her role in RKO's Sudden Fear, Crawford appeared in films ranging from the camp western film Johnny Guitar ( 1954 ) to the drama Autumn Leaves ( 1956 ), opposite a young Cliff Robertson, a movie which showed Crawford in a great many kissing scenes which shocked some audiences.

After months of trade screenings, Paramount introduced VistaVision to the public at Radio City Music Hall on October 14, 1954, with their first film shot in the process, White Christmas.