After dialysis the sample was centrifuged and the supernatant placed on a Af cm column of EEAE-cellulose equilibrated with starting buffer.

After starting his writing career by writing for " true confession " style pulp magazines like True Story, van Vogt decided to switch to writing something he enjoyed, science fiction.

After leaving school at 17, Ayckbourn's career took several temporary jobs in various places before starting a temporary job at the Scarborough Library Theatre, where he was introduced to the artistic director, Stephen Joseph.

After the tumultuous and disappointing 2007 season, the Falcons made a number of moves, hiring a new General Manager and head coach, drafting a new starting quarterback, and signing a starting running back.

After rallying his troops near Schwennenbach – well beyond their starting point – Eugene prepared to launch a second attack, led by the second-line squadrons under the Duke of Württemberg-Teck.

After starting the 1954 campaign with a two-game split against the Tigers in Detroit, the Orioles returned to Baltimore on April 15 to a welcoming parade that wound through the streets of downtown, with an estimated 350, 000 spectators lining the route.

After the victory Boston's owner sold its star pitcher, Babe Ruth, to the New York Yankees to help funding of a Broadway play, and thus starting a tale of futility which would last 86 years, known as Curse of the Bambino.

After starting the season 15 – 35, though, the team had some success later in the year, going a respectable 30 – 28 in August and September as the youngsters became more experienced.

After defeating, but not subjugating, the Kingdom of Scotland, he declared himself rightful heir to the French throne in 1338, starting what would be known as the Hundred Years ' War.

After passing his examinations in 1752, he soon progressed through the merchant navy ranks, starting with his promotion in that year to mate aboard the collier brig Friendship.

After various Korean border conflicts, war broke out with U. S .- allied South Korea in 1950, starting the Korean War.

After cutting out the hatchlings may lie in their eggshells for hours before starting to dig out of the nest.

After being sent on a lengthy tour of Europe starting 1927 – including London, Paris and towns in Germany – during which he demonstrated his invention to full audiences, Theremin found his way to the United States, arriving on 30 December 1927 with his first wife Katia.

After the 1997 death of Scott Krueger, a new member at the Phi Gamma Delta fraternity, MIT required all freshmen to live in the dormitory system starting in 2002.

After starting a collaboration on heart applications with Robert Weiss at Johns Hopkins, Bottomley returned to the university in 1994, as Russell Morgan Professor and Director of the MR Research Division.

After the introduction of the motel, auto camps continued in popularity through the Depression years and after World War II, their popularity finally starting to diminish with the construction of freeways and changes in consumer demands.

After starting 3 – 7 in 1994, the Giants won their final six games to finish 9 – 7 but missed the playoffs.

After starting 5 – 0 in the 2009 season, New York lost to the likewise undefeated New Orleans Saints at the Superdome 48 – 27, beginning a four game losing streak, in which they lost to the Arizona Cardinals 24 – 17, the San Diego Chargers 21 – 20 and the Philadelphia Eagles 40 – 17.

After La Russa retired as manager of the Cardinals in 2011, Smith became active in the organization again, starting with his stint as a special instructor for the team's 2012 spring training camp.

After everyone has made their decision, the replacement cards are dealt starting at the dealer's left as usual.

After reaggravating a hamstring injury during Game 3 of the 2007 ALDS, Joe Torre removed Roger Clemens from the team's starting rotation.

After a while, Prokofiev's mother felt that the isolation in Sontsovka was restricting his further musical development, yet his parents hesitated over starting their son on a musical career at such an early age.

After the Welsh victory at the battle of Llwchwr in January 1136 and the successful ambush of Richard Fitz Gilbert de Clare in April, south Wales rose in rebellion, starting in east Glamorgan and rapidly spreading across the rest of south Wales during 1137.

After finishing its work on the first car, the engine installation crew can begin working on the second car.

After the engine has been installed on the second car, the second car moves to the hood assembly.

After the first engine powered bus of 1895.

After graduation he was employed as a refrigerator engineer, but his true love lay in engine design.

After releasing its atmospheric probe on 13 July 1995, the Galileo orbiter became the first man-made satellite of Jupiter at 00: 27 UT on 8 December 1995 when it fired its main engine to enter a 198-day parking orbit.

After the VZ, the High Feature engine powered the all-new VE Commodore.

After much experimentation, Watt demonstrated that about three quarters of the heat of the steam was being wasted-consumed in heating the engine cylinder on every cycle.

After a generally unsuccessful season in 1965, Honda revised their 1-litre engine completely.

After booster engine cutoff ( BECO ), the LES was no longer needed and was separated from the spacecraft by a solid fuel, 800 lbf ( 3. 6 kN ) thrust jettison rocket that fired for 1. 5 seconds.

After a few years of advertising Mobil gasolines as " Megatane "- rated and as " High Energy " gasolines, Mobil began, in 1966, to promote both its Regular and Premium fuels as " Detergent Gasolines ," due to the inclusion of additives designed to clean carburetors and various internal engine parts.

After a new steam engine had an accident ( cracked pipe weld ) his experiments were banned by the Austro-Hungarian police as dangerous.

After deciding that the mid-engine layout didn't allow enough room in the passenger compartment, a front engine / rear wheel drive layout was chosen.

After years of development, Mazda's first Wankel engine car was the 1967 Cosmo 110S.

After the founding of the Dodge Brothers Company by Horace and John Dodge in 1900, the Detroit-based company quickly found work producing precision engine and chassis components for the city ’ s burgeoning number of automobile firms.

After the launch of the Mozilla project in early 1998, the new layout engine code was released under an open-source license.

After personal computers became more widespread, the first laser printer intended for a mass market was the HP LaserJet 8ppm, released in 1984, using a Canon engine controlled by HP software.

After major accidents at the 1955 24 Hours of Le Mans and the 1957 Mille Miglia the power of sports cars was curbed with a 3 litre engine capacity limit applied to them in the World Championship from 1958.

After the lease ran out, Tucker quit Ford and returned to the police force again, but in his first winter back he was banned from driving police vehicles by the force after using a blowtorch to cut a hole in the dashboard of a cruiser to allow engine heat to warm the cabin.

After the attack was determined to have originated in China, the company stated that it would no longer agree to censor its search engine in China and may exit the country altogether.

After the introduction of Tatra 11 and Tatra 12 cars with their distinctive backbone tube design and swing axles, Tatra introduced its first truck on the same basis, the light utility Tatra 13 powered by 2-cylinder air-cooled petrol engine with power output 8. 8 kW ( 12 hp ) and cargo capacity.

After this year, all GM models would go through " downsizing " and shrink in length, width, weight and available engine size.

After calming him down, Ferris comes up with a plan to run the car's engine in reverse inside Cameron's father's hillside garage, hoping to undo the mileage on the odometer.

After takeoff the left engine lost power.