Other chemicals, known as DNA intercalators, fit into the gap between adjacent bases on a single strand and induce frameshift mutations by " masquerading " as a base, causing the DNA replication machinery to skip or insert additional nucleotides at the intercalated site.

3-The mobile plasmid is nicked and a single strand of DNA is then transferred to the recipient cell.

In the most common usage, β strand refers to a single continuous stretch of amino acids adopting an extended conformation and involved in backbone hydrogen bonds to at least one other strand ; by contrast, a β sheet refers to an assembly of at least two such β strands that are hydrogen-bonded ( or H-bonded ) to each other.

This enzyme operates on a single strand of mRNA, generating its complementary DNA based on the pairing of RNA base pairs ( A, U, G and C ) to their DNA complements ( T, A, C and G respectively ).

# A poly-T oligonucleotide primer is hybridized onto the poly-A tail of the mature mRNA template, or random hexamer primers can be added which contain every possible 6 base single strand of DNA and can therefore hybridize anywhere on the RNA ( Reverse transcriptase requires this double-stranded segment as a primer to start its operation.

The denaturation of nucleic acids such as DNA due to high temperatures is the separation of a double strand into two single strands, which occurs when the hydrogen bonds between the strands are broken.

Alphasatellites are small circular single strand DNA viruses that require a begomovirus for transmission.

Each single strand of DNA is a chain of four types of nucleotides having the bases: adenine, cytosine, guanine, and thymine ( commonly noted as A, C, G & T ).

DNA strands have a directionality, and the different ends of a single strand are called the " 3 ' ( three-prime ) end " and the " 5 ' ( five-prime ) end " with the direction of the naming going 5 prime to the 3 prime region.

The nucleotides on a single strand can be used to reconstruct nucleotides on a newly synthesized partner strand.

In the single stranded DNA viruses-a group that includes the circoviruses, the geminiviruses, the parvoviruses and others-and also the many phages and plasmids that use the rolling circle replication ( RCR ) mechanism, the RCR endonuclease creates a nick the genome strand ( single stranded viruses ) or one of the DNA strands ( plasmids ).

When this is complete, a single nick on the leading strand and several nicks on the lagging strand can be found.

In it, the assassin's sword was seen hanging by a single strand of horsehair above Le Peletier's body, a concept inspired by the proverbial ancient tale of the sword of Damocles, which illustrated the insecurity of power and position.

The braid pattern ensures that each wire strand spends the same amount of its length on the outside of the braid, so skin effect distributes the current equally between the strands, resulting in a larger cross-sectional conduction area than an equivalent single wire.

In the 2001 animated film Final Fantasy: The Spirits Within, every strand of hair on a character's head was independent ; real-time needs would likely force them to be treated as a single unit.

Other microminiatures include violins, dancers, a crucifix ( made of a single strand of the artist's hair and gold ); characters like Donald Duck, Pinocchio, Snow White and the Seven Dwarfs ; a self-portrait ; a golf player and a baseball player swinging his bat.

3-The mobile plasmid is nicked and a single strand of DNA is then transferred to the recipient cell.

The entire globular granule may contain approximately 30, 000 glucose units. A view of the atom ic structure of a single branched strand of glucose units in a glycogen molecule.

RNA polymerase reads the DNA strand from 3-prime ( 3 ') end to the 5-prime ( 5 ') end, while it synthesizes a single strand of messenger RNA in the 5 '- to-3 ' direction.

The Russian abacus, the schoty ( счёты ), usually has a single slanted deck, with ten beads on each wire ( except one wire which has four beads, for quarter-ruble fractions.

In rural areas the grid's cost and impacts can be reduced by using single wire earth return systems ( for example, the MALT-system ).

The NSA did not dictate a single wire!

A simple example of this is a single loop of wire with some constant current flowing through it.

The bare gauze was easily damaged, and once just a single wire broke or rusted away, the lamp became unsafe.

Depending on the fashion at the time of construction it is either a single standard carriageway road, usually wide, or a 2 + 1 road, a wide road with two lanes in one direction and one in the other with a steel wire barrier in between, or sometimes a motorway with two lanes in each direction.

On August 8, 1890 he established communications between Paris, Vannes, and Lorient over a single wire.

On April 27, 1894, he established communications between the Paris stock exchange and the Milan stock exchange, again over a single wire, using his new invention, the retransmitter.

Some hairpins are a single straight pin, but modern versions are more likely to be constructed from different lengths of wire that are bent in half with a u-shaped end and a few kinks along the two opposite portions.

Instead of a single solid conductor, litz wire consists of several smaller wire strands that carry the current.

The biggest single cause of casualties in World War I was actually artillery, but combined with wire entanglements, machine guns earned a fearsome reputation.

A twisted pair line rejects electromagnetic interference ( EMI ) and crosstalk better than a single wire or an untwisted pair.

Early telephones used a single wire for the subscriber's line, with ground return used to complete the circuit ( as used in telegraphs ).

Disadvantages of single wire operation such as crosstalk and hum from nearby AC power wires had already led to the use of twisted pairs and, for long distance telephones, four-wire circuits.

A single volt is defined as the difference in electric potential across a wire when an electric current of one ampere dissipates one watt of power.

In 1837 however, Carl August von Steinheil of Munich, Germany found that by connecting one leg of the apparatus at each station to metal plates buried in the ground, he could eliminate one wire and use a single wire for telegraphic communication.

A wire is a single, usually cylindrical, flexible strand or rod of metal.

Stranded wire might seem to reduce this effect, since the total surface area of the strands is greater than the surface area of the equivalent solid wire, but ordinary stranded wire does not reduce the skin effect because all the strands are short-circuited together and behave as a single conductor.

Others, which are reached by walking up a single flight of stairs, have balconies.

According to a single unsupported piece of recent research, refugees from the collapsed Assyrian Empire claim to have reached the region of Lake Chad and founded the kingdoms of Kanem and Kebbi.

Flatt and Scruggs subsequently cut their own version of the theme ( with Flatt singing ) for Columbia Records ; released as a single, it reached # 44 on Billboard Hot 100 pop music chart and # 1 on the Billboard Hot Country chart ( the lone country chart-topper for the duo ).

For example if a runner on first base reached third base on a single, it would count as a steal.

The album's first single, " Mean to Me ", reached the Australian Kent Music Report Singles Chart top 30 in June.

The single, " Don't Dream It's Over ", was released in December 1986 and reached number one on the New Zealand Singles Chart and number eight in Australia.

The first single " Better Be Home Soon " peaked at number two on both Australian and New Zealand singles charts and reached top 50 In the US, though the following four singles were less successful.

The single reached No. 38 on the country charts.

The same year saw the release of " Kung Fu Fighting ", performed by Carl Douglas and produced by Biddu, which reached # 1 in both the U. K. and U. S., and became the best-selling single of the year and one of the best-selling singles of all time with eleven million records sold worldwide, helping to popularize disco music to a great extent.

Her first country single, " Dumb Blonde " ( one of the few songs during this era, that she recorded but did not write ), reached number twenty-four on the country music charts in 1967, followed the same year with Something Fishy, which went to number seventeen.

Though it topped the country albums charts, it stalled at No. 71 on the pop albums chart ; the album's single, " Light of a Clear Blue Morning " only reached No. 87 on the Hot 100.

She continued to have hits with " Heartbreaker " ( 1978 ), " Baby I'm Burning " and " You're the Only One " ( both 1979 ), all of which charted in the pop singles Top 40, and all of which also topped the country-singles chart ; 1979's " Sweet Summer Lovin '" became the first Parton single in two years to not top the country singles chart ( though it still nonetheless reached the top ten ).

A duet with Ricky Van Shelton, " Rockin ' Years " ( 1991 ) reached number one but Parton's greatest commercial fortune of the decade came when Whitney Houston recorded " I Will Always Love You " for the soundtrack of the feature film The Bodyguard ( 1992 ); both the single and the album were massively successful.

Despite these forebodings, Low yielded the UK number three single " Sound and Vision ", and its own performance surpassed that of Station to Station in the UK chart, where it reached number two.

" American Pie " reached number one on the U. S. Billboard magazine charts for four weeks in 1972, and remains McLean's most successful single release.

In Germany, " Only Time " re-entered the German single charts at number 1 and the album A Day Without Rain reached number one some weeks later.

Montreal singer Nancy Martinez's 1986 single " For Tonight " would become the first Canadian freestyle single to reach the Top 40 on the Billboard Hot 100 chart, while the Montreal girl group 11: 30 reached the Canadian chart with " Ole Ole " in 2000.

That same year " The Red Strokes " became Brooks's first single to make the pop top 40 in the UK, reaching a high of No. 13 ; it was followed by " Standing Outside The Fire ", which reached No. 23.

Each barrel fired a single shot when it reached a certain point in the cycle, after which it ejected the spent cartridge, loaded a new round, and in the process, cooled down somewhat.

The group is best known for its 1992 hit single " Jump Around ", which reached number 3 in the United States, number 6 in Ireland and number 8 in the United Kingdom.

The initial single cell combination is already dividing and by the time of entry into the uterus, the egg might have already reached the blastocyst stage.

As a result Brown's record sales and concerts in the United States reached a lull in 1973 as he failed to land a number-one R & B single that year.

His second album, 1986's The Secret Value of Daydreaming was panned by critics, but reached number 32 on the Billboard Magazines album chart, and produced the single " Stick Around ", which was his first # 1 single on the U. S. Album Rock Tracks chart.