After dialysis the sample was centrifuged and the supernatant placed on a Af cm column of EEAE-cellulose equilibrated with starting buffer.

After starting his writing career by writing for " true confession " style pulp magazines like True Story, van Vogt decided to switch to writing something he enjoyed, science fiction.

After leaving school at 17, Ayckbourn's career took several temporary jobs in various places before starting a temporary job at the Scarborough Library Theatre, where he was introduced to the artistic director, Stephen Joseph.

After the tumultuous and disappointing 2007 season, the Falcons made a number of moves, hiring a new General Manager and head coach, drafting a new starting quarterback, and signing a starting running back.

After rallying his troops near Schwennenbach – well beyond their starting point – Eugene prepared to launch a second attack, led by the second-line squadrons under the Duke of Württemberg-Teck.

After starting the 1954 campaign with a two-game split against the Tigers in Detroit, the Orioles returned to Baltimore on April 15 to a welcoming parade that wound through the streets of downtown, with an estimated 350, 000 spectators lining the route.

After the victory Boston's owner sold its star pitcher, Babe Ruth, to the New York Yankees to help funding of a Broadway play, and thus starting a tale of futility which would last 86 years, known as Curse of the Bambino.

After starting the season 15 – 35, though, the team had some success later in the year, going a respectable 30 – 28 in August and September as the youngsters became more experienced.

After defeating, but not subjugating, the Kingdom of Scotland, he declared himself rightful heir to the French throne in 1338, starting what would be known as the Hundred Years ' War.

After passing his examinations in 1752, he soon progressed through the merchant navy ranks, starting with his promotion in that year to mate aboard the collier brig Friendship.

After various Korean border conflicts, war broke out with U. S .- allied South Korea in 1950, starting the Korean War.

After cutting out the hatchlings may lie in their eggshells for hours before starting to dig out of the nest.

After being sent on a lengthy tour of Europe starting 1927 – including London, Paris and towns in Germany – during which he demonstrated his invention to full audiences, Theremin found his way to the United States, arriving on 30 December 1927 with his first wife Katia.

After the 1997 death of Scott Krueger, a new member at the Phi Gamma Delta fraternity, MIT required all freshmen to live in the dormitory system starting in 2002.

After starting a collaboration on heart applications with Robert Weiss at Johns Hopkins, Bottomley returned to the university in 1994, as Russell Morgan Professor and Director of the MR Research Division.

After the introduction of the motel, auto camps continued in popularity through the Depression years and after World War II, their popularity finally starting to diminish with the construction of freeways and changes in consumer demands.

After starting 3 – 7 in 1994, the Giants won their final six games to finish 9 – 7 but missed the playoffs.

After starting 5 – 0 in the 2009 season, New York lost to the likewise undefeated New Orleans Saints at the Superdome 48 – 27, beginning a four game losing streak, in which they lost to the Arizona Cardinals 24 – 17, the San Diego Chargers 21 – 20 and the Philadelphia Eagles 40 – 17.

After La Russa retired as manager of the Cardinals in 2011, Smith became active in the organization again, starting with his stint as a special instructor for the team's 2012 spring training camp.

After everyone has made their decision, the replacement cards are dealt starting at the dealer's left as usual.

After reaggravating a hamstring injury during Game 3 of the 2007 ALDS, Joe Torre removed Roger Clemens from the team's starting rotation.

After starting the engine with a known setting that allowed it to idle, the air valve was opened until maximum engine speed was obtained.

After a while, Prokofiev's mother felt that the isolation in Sontsovka was restricting his further musical development, yet his parents hesitated over starting their son on a musical career at such an early age.

After the Welsh victory at the battle of Llwchwr in January 1136 and the successful ambush of Richard Fitz Gilbert de Clare in April, south Wales rose in rebellion, starting in east Glamorgan and rapidly spreading across the rest of south Wales during 1137.

After a decade of double-digit growth, Armenia's economy declined by 14. 4 percent in 2009.

After the victory of the PdL in the 2008 general election, AN was merged into the PdL in early 2009.

After the second terminal of International Airport Sofia was built the total number of passengers rose and reached 3 230 696 in 2008, and in April 2011 Airport Sofia serviced 282 694 passengers, 13 % more than the same period of 2009, when the record was 250 000 passengers.

After that, other films included The Triangle in 2001, The Triangle ( a TV miniseries ) in 2005, and Triangle in 2009.

After a poor start ( 19 – 28 ) to the 2009 season, Clint Hurdle was fired on May 29.

After spending almost the entire 2009 / 2010 season in the top six of League One, Charlton were defeated in the Football League One play-offs semi-final second leg on penalties to Swindon Town, condemning Charlton to another season in the third tier of English Football.

After Cowboys Stadium was opened in 2009, the Cowboys turned over the facility to the City of Irving.

After sixteen years, Microsoft discontinued the Encarta line of products in 2009.

After sustaining severe damage from this type of tackle during the 2009 AFL season, Brent Harvey called for this type of tackle to be banned.

*" The Power of Love ", by After the Anthems ( 2009 )

After the inauguration of Jacob Zuma as South Africa's president in May 2009 de Klerk said he is optimistic that Zuma and his government can " confound the prophets of doom ".

After a slight slowdown of economy in 2009 (- 3. 8 %) country recovered shortly with 6. 3 % GDP real growth 2010.

After passing the initial aptitude test, he was to undertake a month-long course ; if he passed that, he would begin full flight training in early 2009.

After King Abdullah II's accession to the throne in 1999, liberal economic policies were introduced that resulted in a boom lasting for a decade, continuing through 2009.

* 2009 – After 65 straight days of civil disobedience, at least 31 people are killed in clashes between security forces and indigenous people near Bagua, Peru.

After Robinson's death, his widow founded the Jackie Robinson Foundation, of which she remains an officer as of 2009.

After serving seven years, about half of her sentence, Olson was released from prison on March 17, 2009, and will serve her parole in Minnesota.

After talks, on 4 March 2009 five parties confirmed their participation in the coalition: New Era, People's Party, Union of Greens and Peasants, For Fatherland and Freedom / LNNK, and Civic Union.

After finishing as runner-up to Chelsea in the 2009 – 10 season, United achieved a record 19th league title in 2010 – 11, securing the championship with a 1 – 1 away draw against Blackburn Rovers on 14 May 2011.

After having lost $ 4. 3 billion from 2007 to 2009, the company was divided into two independent public companies, Motorola Mobility and Motorola Solutions on January 4, 2011.

After nearly 70 years of use, the vast majority of over-the-air NTSC transmissions in the United States were turned off on June 12, 2009 and August 31, 2011 in Canada and most other NTSC markets.

After a lengthy investigation conducted by the National Football League's security department, the league alleged on March 2, 2012 that 22 to 27 defensive players on the New Orleans Saints maintained a " pay for performance " program that included " bounty " payments administered by then-defensive coordinator Gregg Williams during the 2009, 2010 and 2011 seasons.

After the Mumbai incident of 2009 and subsequent standoff with India, several brigades were moved back east.

Gillick retired as general manager after the 2008 season and was succeeded by one of his assistants, Ruben Amaro, Jr. After adding outfielder Raúl Ibañez to replace the departed Pat Burrell, the Phillies retained the majority of their core players for the 2009 season.