After dialysis the sample was centrifuged and the supernatant placed on a Af cm column of EEAE-cellulose equilibrated with starting buffer.

After starting his writing career by writing for " true confession " style pulp magazines like True Story, van Vogt decided to switch to writing something he enjoyed, science fiction.

After leaving school at 17, Ayckbourn's career took several temporary jobs in various places before starting a temporary job at the Scarborough Library Theatre, where he was introduced to the artistic director, Stephen Joseph.

After the tumultuous and disappointing 2007 season, the Falcons made a number of moves, hiring a new General Manager and head coach, drafting a new starting quarterback, and signing a starting running back.

After rallying his troops near Schwennenbach – well beyond their starting point – Eugene prepared to launch a second attack, led by the second-line squadrons under the Duke of Württemberg-Teck.

After starting the 1954 campaign with a two-game split against the Tigers in Detroit, the Orioles returned to Baltimore on April 15 to a welcoming parade that wound through the streets of downtown, with an estimated 350, 000 spectators lining the route.

After the victory Boston's owner sold its star pitcher, Babe Ruth, to the New York Yankees to help funding of a Broadway play, and thus starting a tale of futility which would last 86 years, known as Curse of the Bambino.

After starting the season 15 – 35, though, the team had some success later in the year, going a respectable 30 – 28 in August and September as the youngsters became more experienced.

After defeating, but not subjugating, the Kingdom of Scotland, he declared himself rightful heir to the French throne in 1338, starting what would be known as the Hundred Years ' War.

After passing his examinations in 1752, he soon progressed through the merchant navy ranks, starting with his promotion in that year to mate aboard the collier brig Friendship.

After various Korean border conflicts, war broke out with U. S .- allied South Korea in 1950, starting the Korean War.

After cutting out the hatchlings may lie in their eggshells for hours before starting to dig out of the nest.

After being sent on a lengthy tour of Europe starting 1927 – including London, Paris and towns in Germany – during which he demonstrated his invention to full audiences, Theremin found his way to the United States, arriving on 30 December 1927 with his first wife Katia.

After the 1997 death of Scott Krueger, a new member at the Phi Gamma Delta fraternity, MIT required all freshmen to live in the dormitory system starting in 2002.

After starting a collaboration on heart applications with Robert Weiss at Johns Hopkins, Bottomley returned to the university in 1994, as Russell Morgan Professor and Director of the MR Research Division.

After the introduction of the motel, auto camps continued in popularity through the Depression years and after World War II, their popularity finally starting to diminish with the construction of freeways and changes in consumer demands.

After starting 3 – 7 in 1994, the Giants won their final six games to finish 9 – 7 but missed the playoffs.

After starting 5 – 0 in the 2009 season, New York lost to the likewise undefeated New Orleans Saints at the Superdome 48 – 27, beginning a four game losing streak, in which they lost to the Arizona Cardinals 24 – 17, the San Diego Chargers 21 – 20 and the Philadelphia Eagles 40 – 17.

After La Russa retired as manager of the Cardinals in 2011, Smith became active in the organization again, starting with his stint as a special instructor for the team's 2012 spring training camp.

After everyone has made their decision, the replacement cards are dealt starting at the dealer's left as usual.

After reaggravating a hamstring injury during Game 3 of the 2007 ALDS, Joe Torre removed Roger Clemens from the team's starting rotation.

After starting the engine with a known setting that allowed it to idle, the air valve was opened until maximum engine speed was obtained.

After a while, Prokofiev's mother felt that the isolation in Sontsovka was restricting his further musical development, yet his parents hesitated over starting their son on a musical career at such an early age.

After the Welsh victory at the battle of Llwchwr in January 1136 and the successful ambush of Richard Fitz Gilbert de Clare in April, south Wales rose in rebellion, starting in east Glamorgan and rapidly spreading across the rest of south Wales during 1137.

After a while, we became aware that the money was disappearing as fast as we replenished it.

After that he was never known to run or even walk fast.

After Lazarus Saturday comes Palm Sunday, Holy Week, and finally Pascha itself, and the fast is broken immediately after the Paschal Divine Liturgy.

After the internal affairs stabilized, the still mainly agrarian economy grew relatively fast.

After this metamorphosis, hellbenders have to be able to absorb oxygen through the folds in their skin, which is largely behind the need for fast moving, oxygenated water.

" After Jonah walked for a day across Nineveh, the people of Nineveh began to believe his word and proclaimed a fast.

After the Requiem Mass and service, his remains were brought upstream to Rhöndorf on the Rhine aboard Kondor, with Seeadler and Sperber as escorts, three Jaguar class fast attack craft of the German Navy, " past the thousands who stood in silence on both banks of the river ".

After the 1923 Great Kantō earthquake, kimono wearers often became victims of robbery because they could not run very fast due to the restricting nature of the kimono on the body and geta clogs.

After the waterfall the river enters a gorge where there is very fast whitewater with class-4 rapids ; it is often used for whitewater rafting although it is not considered to be of the same quality as the Tena River and is therefore less popular for the sport.

After his fast start, Clemente finished his rookie season with an average of. 255, owing at least in part to the recurrence, or perhaps persistence, of the counterproductive batting tics noticed three years before by Dodger scout Al Campanis-and, more to the point, the fact that National League pitchers were noticing as well.

After the Great Storm of 1703, Anne declared a general fast to implore God " to pardon the crying sins of this nation which had drawn down this sad judgement ".

This discharge takes no more than a few microseconds, and is able to reach accelerations of about 40, 000 g. Recent research suggests the process to occur as fast as 700 nanoseconds, thus reaching an acceleration of up to 5, 410, 000 g. After penetration, the toxic content of the nematocyst is injected into the target organism, then the sessile cnidarian to devour it.

After the war, railroads rejuvenated overworked and neglected fleets with fast and often luxurious streamliners – epitomized by the Super Chief and California Zephyr – which inspired the last major resurgence in passenger rail travel.

After an overnight fast, blood is drawn and then 50 grams of lactose ( in aqueous solution ) is swallowed.

After an initial rush thousands of supporters entered the stadium " steadily at a fast walk ".

After prodding by their daughter Ida, who " perceived the need to act fast ", and with help from Alfred Barr of the New York Museum of Modern Art, Chagall was saved by having his name added to the list of prominent artists whose lives were at risk and who the United States should try to extricate.

After evaluating the performance of three different fast foods as rocket fuels ( combined with nitrous oxide ), the winning fuel was used to propel the C5 in a drag race with an electric scooter.

After an unsuccessful attempt at playing tennis himself, Hammer gets rid of his sleep deficit by spending all day in his room, fast asleep, with " old junior " — his gun — close to him.

After the Second World War there was a fast increase of population from 3, 700 to 12, 500 inhabitants in Eersel.

After his release he formed a bogus hydroponics business and swindled a friend out of $ 25, 000 who had hoped to receive a fast investment return to pay for his dying wife's health care.

After six years at the Inner Temple he was called to the Bar on 20 April 1578, a remarkably fast rate of progress given the process of legal education at the time, which normally required eight years of study.

After suffering under a tyrannous pure democracy which had the power to create laws so fast that no thought could be given to the effects, the sentients of the galaxy found the need for a Bureau of Sabotage ( BuSab ) to slow the wheels of government, thereby preventing it from legislating recklessly.

After careful study of potential battlefields, in October 1597 Admiral Yi lured the Japanese fleet into the Myeongnyang Strait, by sending a fast warship near the Japanese naval base and luring the Japanese fleet out of anchorage.

After that comes a fast and very demanding single-line fugue that leads to the powerful end.