After dialysis the sample was centrifuged and the supernatant placed on a Af cm column of EEAE-cellulose equilibrated with starting buffer.

After starting his writing career by writing for " true confession " style pulp magazines like True Story, van Vogt decided to switch to writing something he enjoyed, science fiction.

After leaving school at 17, Ayckbourn's career took several temporary jobs in various places before starting a temporary job at the Scarborough Library Theatre, where he was introduced to the artistic director, Stephen Joseph.

After the tumultuous and disappointing 2007 season, the Falcons made a number of moves, hiring a new General Manager and head coach, drafting a new starting quarterback, and signing a starting running back.

After rallying his troops near Schwennenbach – well beyond their starting point – Eugene prepared to launch a second attack, led by the second-line squadrons under the Duke of Württemberg-Teck.

After starting the 1954 campaign with a two-game split against the Tigers in Detroit, the Orioles returned to Baltimore on April 15 to a welcoming parade that wound through the streets of downtown, with an estimated 350, 000 spectators lining the route.

After the victory Boston's owner sold its star pitcher, Babe Ruth, to the New York Yankees to help funding of a Broadway play, and thus starting a tale of futility which would last 86 years, known as Curse of the Bambino.

After starting the season 15 – 35, though, the team had some success later in the year, going a respectable 30 – 28 in August and September as the youngsters became more experienced.

After defeating, but not subjugating, the Kingdom of Scotland, he declared himself rightful heir to the French throne in 1338, starting what would be known as the Hundred Years ' War.

After passing his examinations in 1752, he soon progressed through the merchant navy ranks, starting with his promotion in that year to mate aboard the collier brig Friendship.

After various Korean border conflicts, war broke out with U. S .- allied South Korea in 1950, starting the Korean War.

After cutting out the hatchlings may lie in their eggshells for hours before starting to dig out of the nest.

After being sent on a lengthy tour of Europe starting 1927 – including London, Paris and towns in Germany – during which he demonstrated his invention to full audiences, Theremin found his way to the United States, arriving on 30 December 1927 with his first wife Katia.

After the 1997 death of Scott Krueger, a new member at the Phi Gamma Delta fraternity, MIT required all freshmen to live in the dormitory system starting in 2002.

After starting a collaboration on heart applications with Robert Weiss at Johns Hopkins, Bottomley returned to the university in 1994, as Russell Morgan Professor and Director of the MR Research Division.

After the introduction of the motel, auto camps continued in popularity through the Depression years and after World War II, their popularity finally starting to diminish with the construction of freeways and changes in consumer demands.

After starting 3 – 7 in 1994, the Giants won their final six games to finish 9 – 7 but missed the playoffs.

After starting 5 – 0 in the 2009 season, New York lost to the likewise undefeated New Orleans Saints at the Superdome 48 – 27, beginning a four game losing streak, in which they lost to the Arizona Cardinals 24 – 17, the San Diego Chargers 21 – 20 and the Philadelphia Eagles 40 – 17.

After La Russa retired as manager of the Cardinals in 2011, Smith became active in the organization again, starting with his stint as a special instructor for the team's 2012 spring training camp.

After everyone has made their decision, the replacement cards are dealt starting at the dealer's left as usual.

After reaggravating a hamstring injury during Game 3 of the 2007 ALDS, Joe Torre removed Roger Clemens from the team's starting rotation.

After starting the engine with a known setting that allowed it to idle, the air valve was opened until maximum engine speed was obtained.

After a while, Prokofiev's mother felt that the isolation in Sontsovka was restricting his further musical development, yet his parents hesitated over starting their son on a musical career at such an early age.

After the Welsh victory at the battle of Llwchwr in January 1136 and the successful ambush of Richard Fitz Gilbert de Clare in April, south Wales rose in rebellion, starting in east Glamorgan and rapidly spreading across the rest of south Wales during 1137.

After a personally successful 1990 NSWRL season in which he played fullback in all three Origin games for NSW which saw The Blues win the series for the first time since 1986 and after scoring 13 tries in 20 games for Cronulla, he was selected for the 17th Kangaroo Tour of Great Britain and France where he played in all five tests on tour against Great Britain and France.

After Slater's second suspension, Melbourne Storm coach Craig Bellamy decided to give Slater some warm up matches for his comeback into first grade with both North Sydney Bears ( Melbourne's feeder club in the NSWRL Premier League ) and Norths Devils ( Melbourne's feeder club in the Queensland Cup ).

After moving from Brisbane's Wests club to play for the defending NSWRL premiers Manly-Warringah starting in 1979, he played in Grand Finals with Manly in 1982 and 1983 but in 1983 was voted the Dally M Player's Player of the year, as well as Representative Player of the Year and later enjoyed success in leading Manly to victory in the 1987 Grand Final.

After leading North Melbourne to the Ansett Cup Premiership in the pre-season, Carey's reputation as the games number one player continued to grow early in 1995.

After the 2004 elections President Thabo Mbeki offered Buthelezi the Deputy Presidency, which he refused, as in exchange the IFP would have to relinquish the Premiership of the IFP-dominated province of KwaZulu-Natal.

After being linked with several Premiership teams, Emerton eventually joined Blackburn Rovers in July 2003 for an undisclosed fee, on a four-year contract, saying, " I've always dreamed of playing in the Premiership so to finally achieve it is great.

After a day of games almost all of which had significant consequences in terms of positions at the top, and at the foot of the table, Saracens found themselves in the Premiership playoffs for the first time, squeezing Wasps in to a rare 5th place position, out of playoff contention.

After just one year spent in the Second League ( 1957 / 58 ) returned to Sarajevo in the first league to date, a full 49 years maintained the status Premiership club.

After Marcelo Loffreda left following the 2007 Rugby World Cup to take up the head coaching job at Guinness Premiership club Leicester Tigers, the UAR spent nearly five months searching for a successor until opting for a two-coach setup, with former Pumas Santiago Phelan and Fabián Turnes taking over.

After the disappointment of the 2008 – 09 Guinness Premiership season, the Warriors made a bright start to the 2009 – 10 Guinness Premiership season.

After winning the play-off against Bedford Blues at Sixways and the both legs of the play-off final, Worcester secured promotion to the Premiership for the 2011-12 season.

After scoring 16 goals in 33 appearances during the 2004 – 05 season, his future was cast into doubt when Southampton were relegated from the English Premiership.

After a successful 2001 season, they narrowly missed out on promotion to the Super League, losing to Widnes 12-24 in the Northern Ford Premiership Grand Final.

After helping the Wanderers to the League Cup and FA Cup Semi Finals, it was now his main aim to get the club into the Premiership.

After Sunderland returned to the Premiership Whitehead found himself playing as a right back, although this was ended when manager Roy Keane signed right backs Phil Bardsley and Pascal Chimbonda for Sunderland, allowing Whitehead to move back into central midfield.

After four seasons in Scotland, it was off to Derby County in the English Premiership.

After the first few matches of 2003 – 04, Birmingham's manager Steve Bruce was describing Cunningham as " on current form ... the best defender in the Premiership ".

After Southampton were relegated in 2005 and failed to be promoted the following season, Niemi decided to return to the Premiership with Fulham, for whom he signed in January 2006.

After merely a year, Huseyn Shaheed Suhrawardy resigned from his Premiership in October 1957.

After a run of 5 goals in 5 matches cemented the club's Premiership status, Dugarry joined the club on a permanent deal but could only manage a single goal in 15 appearances before leaving the club by mutual consent, citing family reasons.

After several successful seasons with Wimbledon Francis moved to Norwich City and made a good impression including helping them reach the Premiership by winning the Championship in season 2003-2004.

After Norwich were relegated at the end of their first season in the Premiership after a disappointing campaign, Francis made the decision to end his two year stay at Carrow Road but remained in the Premiership by securing a transfer to promoted Wigan Athletic.

After being part of Essendon's 1993 Premiership side, Misiti struggled to keep his place in the side in the next two years.

After a few lean seasons during the late 1960s and early 1970s, Croatia won their first NSW Premiership in 1977 with Luka Fabijinic as coach and Atti Aboyni as the team's striker.