After dialysis the sample was centrifuged and the supernatant placed on a Af cm column of EEAE-cellulose equilibrated with starting buffer.

After starting his writing career by writing for " true confession " style pulp magazines like True Story, van Vogt decided to switch to writing something he enjoyed, science fiction.

After leaving school at 17, Ayckbourn's career took several temporary jobs in various places before starting a temporary job at the Scarborough Library Theatre, where he was introduced to the artistic director, Stephen Joseph.

After the tumultuous and disappointing 2007 season, the Falcons made a number of moves, hiring a new General Manager and head coach, drafting a new starting quarterback, and signing a starting running back.

After rallying his troops near Schwennenbach – well beyond their starting point – Eugene prepared to launch a second attack, led by the second-line squadrons under the Duke of Württemberg-Teck.

After starting the 1954 campaign with a two-game split against the Tigers in Detroit, the Orioles returned to Baltimore on April 15 to a welcoming parade that wound through the streets of downtown, with an estimated 350, 000 spectators lining the route.

After the victory Boston's owner sold its star pitcher, Babe Ruth, to the New York Yankees to help funding of a Broadway play, and thus starting a tale of futility which would last 86 years, known as Curse of the Bambino.

After starting the season 15 – 35, though, the team had some success later in the year, going a respectable 30 – 28 in August and September as the youngsters became more experienced.

After defeating, but not subjugating, the Kingdom of Scotland, he declared himself rightful heir to the French throne in 1338, starting what would be known as the Hundred Years ' War.

After passing his examinations in 1752, he soon progressed through the merchant navy ranks, starting with his promotion in that year to mate aboard the collier brig Friendship.

After various Korean border conflicts, war broke out with U. S .- allied South Korea in 1950, starting the Korean War.

After cutting out the hatchlings may lie in their eggshells for hours before starting to dig out of the nest.

After being sent on a lengthy tour of Europe starting 1927 – including London, Paris and towns in Germany – during which he demonstrated his invention to full audiences, Theremin found his way to the United States, arriving on 30 December 1927 with his first wife Katia.

After the 1997 death of Scott Krueger, a new member at the Phi Gamma Delta fraternity, MIT required all freshmen to live in the dormitory system starting in 2002.

After starting a collaboration on heart applications with Robert Weiss at Johns Hopkins, Bottomley returned to the university in 1994, as Russell Morgan Professor and Director of the MR Research Division.

After the introduction of the motel, auto camps continued in popularity through the Depression years and after World War II, their popularity finally starting to diminish with the construction of freeways and changes in consumer demands.

After starting 3 – 7 in 1994, the Giants won their final six games to finish 9 – 7 but missed the playoffs.

After starting 5 – 0 in the 2009 season, New York lost to the likewise undefeated New Orleans Saints at the Superdome 48 – 27, beginning a four game losing streak, in which they lost to the Arizona Cardinals 24 – 17, the San Diego Chargers 21 – 20 and the Philadelphia Eagles 40 – 17.

After La Russa retired as manager of the Cardinals in 2011, Smith became active in the organization again, starting with his stint as a special instructor for the team's 2012 spring training camp.

After everyone has made their decision, the replacement cards are dealt starting at the dealer's left as usual.

After reaggravating a hamstring injury during Game 3 of the 2007 ALDS, Joe Torre removed Roger Clemens from the team's starting rotation.

After starting the engine with a known setting that allowed it to idle, the air valve was opened until maximum engine speed was obtained.

After a while, Prokofiev's mother felt that the isolation in Sontsovka was restricting his further musical development, yet his parents hesitated over starting their son on a musical career at such an early age.

After the Welsh victory at the battle of Llwchwr in January 1136 and the successful ambush of Richard Fitz Gilbert de Clare in April, south Wales rose in rebellion, starting in east Glamorgan and rapidly spreading across the rest of south Wales during 1137.

After the release of the Apple Lisa computer in January 1983, Apple invested considerable effort in the development of a local area networking ( LAN ) system for the machines.

In 1983, the American National Standards Institute formed a committee, X3J11, to establish a standard specification of C. After a long and arduous process, the standard was completed in 1989 and ratified as ANSI X3. 159-1989 " Programming Language C ." This version of the language is often referred to as " ANSI C ", or sometimes " C89 " ( to distinguish it from C99 ).

After ABBA broke up in 1983, Andersson continued writing music with Ulvaeus.

After a decade as president of the Australian Council of Trade Unions, he entered parliament as a Labor MP at the 1980 federal election and became Prime Minister within three years, leading Labor to victory at four consecutive federal elections: 1983, 1984, 1987 and 1990.

After his death, film historians Kevin Brownlow and David Gill examined out-takes from the Mutual films and presented their findings in a three-part documentary Unknown Chaplin ( 1983 ).

Examples include Arsenic and Old Lace ( 1944 ), Monsieur Verdoux ( 1947 ), Kind Hearts and Coronets ( 1949 ), The Ladykillers ( 1955 ), Dr. Strangelove or: How I Learned to Stop Worrying and Love the Bomb ( 1964 ), The Loved One ( 1965 ), MASH ( 1970 ), Monty Python's The Meaning of Life ( 1983 ), Brazil ( 1985 ), The War of the Roses ( 1989 ), Heathers ( 1989 ), Your Friends & Neighbors ( 1998 ), Keeping Mum ( 2005 ), and Burn After Reading ( 2008 ).

After multiple drafts and many years of discussion, Pope John Paul II promulgated the revised Code of Canon Law ( CIC ) in 1983.

After a further decade of debate, in 1983, JTS voted to admit women for ordination as Conservative rabbis, also without adopting an explanatory responsum.

After the 1983 season, Earnhardt returned to Richard Childress Racing, replacing Ricky Rudd in the # 3.

After Korean Air Lines Flight 007, carrying 269 people, was shot down in 1983 after straying into the USSR's prohibited airspace, in the vicinity of Sakhalin and Moneron Islands, President Ronald Reagan issued a directive making GPS freely available for civilian use, once it was sufficiently developed, as a common good.

After some success at state-level elections, the party won 27 seats with 5. 7 % of the vote in the Bundestag, the lower house of the German parliament, in the 1983 federal election.

After repeated victories in 1983, 1987, 1990 and 1994 he was finally defeated by a landslide that was the biggest on record, for the left in the 1998 federal elections, and was succeeded as Chancellor by Gerhard Schröder of the SPD.

* 1983 – After an emergency landing because of an in-flight fire, twenty-three passengers aboard Air Canada Flight 797 are killed when a flashover occurs as the plane's doors open.

After the Winter of Discontent and the subsequent fall of the Labour government, many corners of the public and media believed that the trade unions were running the Labour Party-an image which Neil Kinnock was keen to shake off after becoming party leader in 1983.

After the 1980 coup d ’ état he served some time in Doe ’ s government until he was sacked in 1983 on accusation of embezzling government funds.

After three election victories, he was defeated by Bob Hawke in the 1983 election and ended his career alienated from his own party.

After the World Series championship, Smith and the Cardinals agreed on a new contract in January 1983 that paid Smith $ 1 million per year.

After Vanni Mulinari's travel to Udine and subsequent arrest by the Italian justice, the Abbé Pierre went to talk in 1983 with Italian President Sandro Pertini to plead Mulinari's cause.

After YMO disbanded in 1983, he produced more solo records, including collaborations with various international artists, through to the 1990s.

After field tests that lasted into 1982, Suitcase SETI was put into use in 1983 with the 26-meter Harvard / Smithsonian radio telescope at Harvard, Massachusetts.

After its first flight in April 1983, Challenger quickly became the workhorse of NASA's Space Shuttle fleet, flying far more missions per year than Columbia.

After seeing an early film of this event, French skiers / surfers Augustin Coppey, Olivier Lehaneur, Olivier Roland and Antoine Yarmola made their first successful attempts during the winter of 1983 in France ( Val Thorens ), using primitive, home-made clones of the Winterstick.

After that date, other authors collaborated with Charteris on books until 1983 ; two additional works produced without Charteris ’ s participation were published in 1997.

After graduating from Timmins High in June 1983, Twain was eager to expand her musical horizons.

After the U. S. Congress prohibited federal funding of the Contras in 1983, the Reagan administration continued to back the Contras by raising money from foreign allies and covertly selling arms to Iran ( then engaged in a vicious war with Iraq ), and channelling the proceeds to the Contras ( see the Iran-Contra Affair ).