After dialysis the sample was centrifuged and the supernatant placed on a Af cm column of EEAE-cellulose equilibrated with starting buffer.

After starting his writing career by writing for " true confession " style pulp magazines like True Story, van Vogt decided to switch to writing something he enjoyed, science fiction.

After leaving school at 17, Ayckbourn's career took several temporary jobs in various places before starting a temporary job at the Scarborough Library Theatre, where he was introduced to the artistic director, Stephen Joseph.

After the tumultuous and disappointing 2007 season, the Falcons made a number of moves, hiring a new General Manager and head coach, drafting a new starting quarterback, and signing a starting running back.

After rallying his troops near Schwennenbach – well beyond their starting point – Eugene prepared to launch a second attack, led by the second-line squadrons under the Duke of Württemberg-Teck.

After starting the 1954 campaign with a two-game split against the Tigers in Detroit, the Orioles returned to Baltimore on April 15 to a welcoming parade that wound through the streets of downtown, with an estimated 350, 000 spectators lining the route.

After the victory Boston's owner sold its star pitcher, Babe Ruth, to the New York Yankees to help funding of a Broadway play, and thus starting a tale of futility which would last 86 years, known as Curse of the Bambino.

After starting the season 15 – 35, though, the team had some success later in the year, going a respectable 30 – 28 in August and September as the youngsters became more experienced.

After defeating, but not subjugating, the Kingdom of Scotland, he declared himself rightful heir to the French throne in 1338, starting what would be known as the Hundred Years ' War.

After passing his examinations in 1752, he soon progressed through the merchant navy ranks, starting with his promotion in that year to mate aboard the collier brig Friendship.

After various Korean border conflicts, war broke out with U. S .- allied South Korea in 1950, starting the Korean War.

After cutting out the hatchlings may lie in their eggshells for hours before starting to dig out of the nest.

After being sent on a lengthy tour of Europe starting 1927 – including London, Paris and towns in Germany – during which he demonstrated his invention to full audiences, Theremin found his way to the United States, arriving on 30 December 1927 with his first wife Katia.

After the 1997 death of Scott Krueger, a new member at the Phi Gamma Delta fraternity, MIT required all freshmen to live in the dormitory system starting in 2002.

After starting a collaboration on heart applications with Robert Weiss at Johns Hopkins, Bottomley returned to the university in 1994, as Russell Morgan Professor and Director of the MR Research Division.

After the introduction of the motel, auto camps continued in popularity through the Depression years and after World War II, their popularity finally starting to diminish with the construction of freeways and changes in consumer demands.

After starting 3 – 7 in 1994, the Giants won their final six games to finish 9 – 7 but missed the playoffs.

After starting 5 – 0 in the 2009 season, New York lost to the likewise undefeated New Orleans Saints at the Superdome 48 – 27, beginning a four game losing streak, in which they lost to the Arizona Cardinals 24 – 17, the San Diego Chargers 21 – 20 and the Philadelphia Eagles 40 – 17.

After La Russa retired as manager of the Cardinals in 2011, Smith became active in the organization again, starting with his stint as a special instructor for the team's 2012 spring training camp.

After everyone has made their decision, the replacement cards are dealt starting at the dealer's left as usual.

After reaggravating a hamstring injury during Game 3 of the 2007 ALDS, Joe Torre removed Roger Clemens from the team's starting rotation.

After starting the engine with a known setting that allowed it to idle, the air valve was opened until maximum engine speed was obtained.

After a while, Prokofiev's mother felt that the isolation in Sontsovka was restricting his further musical development, yet his parents hesitated over starting their son on a musical career at such an early age.

After the Welsh victory at the battle of Llwchwr in January 1136 and the successful ambush of Richard Fitz Gilbert de Clare in April, south Wales rose in rebellion, starting in east Glamorgan and rapidly spreading across the rest of south Wales during 1137.

After walking out to his corral that morning, he'd been amazed to see the dust puff up in front of his feet.

After he had finished the first two volumes of his Lincoln, Sandburg went to work assembling a book of songs out of hobo and childhood days and from the memory of songs others had taught him.

After we got a script and the spots for the songs were blocked out, we'd get together for an hour or so every day.

After baptism Jesus came up out of the water at once, and at that moment heaven opened ; ;

After a nuclear blast, one bureaucrat suggested in those halcyon days, about all you had to do was haul out the broom and sweep off your sidewalks and roof.

After Mr. Jack drove away, Winston went on looking out the window.

After the family has settled in the shelter, the housekeeping rules should be spelled out by the adult in charge.

After figuring out how to regulate the barrels so that they shot to the same point of impact, we fired this little 20-inch-barrel job on my home range and in Marlin's underground test gallery.

After the meal, he and his guests went out to inspect the rig ; ;

After an unspeakable siege, lasting the better part of two months, it was announced that the studio `` owed '' the government a tax debt in excess of eight million dollars while I, who had always remained aloof from such iniquitous practices as paying taxes on the salary I had earned and the little I legally inherited as Morris' helpless relict, was `` stung '' with a personal bill of such astronomical proportions as to `` wipe out '' all but a fraction of my poor, hard-come-by savings.

After blotting out most of the saline around the sections, a drop of Af was layered over each of the sections, allowed to react for 30 minutes, and then washed with PBS for 15 - 30 minutes.

After blotting out most of the liquid around the sections, the latter were mounted in buffered glycerine ( 7 parts glycerine to 3 parts of PBS ).

After the first two were blacked out, the third light was abandoned by a terrified Italian crew, who left their light to shine for nine minutes like an unerring homing beacon until British MP's shot it out.

After all, the henpecked husband with his shrewish wife is a comic figure of long standing, in literature and on the stage, as Dr. Schillinger points out.

After he'd got out fresh liver for Bast, he paused to look at her crouched daintily over her dish.

After Al Paschal gruonded out, Jay Cooke walked and Jim McDaniel singled home McAuliffe.

After playing a splendid first nine holes in 34 -- two strokes under par -- on this fifth and final day of the tournament ( Sunday's fourth round had been washed out by a violent rainstorm when it was only half completed ), Player's game rapidly fell to pieces.

After the sad impatient moment, waiting for comfort which could not come, she slipped out of bed and went to the open window.

After a short time, both George and Donald joined the class with me so they wouldn't feel lonely, and we used to hang a sign on the door of the Brush-off reading out to work.

After the Battle of Gettysburg, Lincoln clearly understood that his military decisions would be more effectively carried out by conveying his orders through his War Secretary or his general-in-chief on to his generals, who resented his civilian interference with their own plans.

After the Civil War, Doubleday mustered out of the volunteer service on August 24, 1865, reverted to the rank of lieutenant colonel, and became the colonel of the 35th U. S. Infantry in September 1867.

After seven days and nights in agony, Alcmene stretched out her arms and called upon Lucina, the goddess of childbirth ( the Roman equivalent of Eileithyia ).

After the pair donned and pressurized their spacesuits and depressurized the Lunar Module cabin, John Young climbed out onto the " porch " of the LM, a small platform above the ladder.

After completing more tasks at the ALSEP, Young and Duke returned to the LM to close out the moonwalk.