After dialysis the sample was centrifuged and the supernatant placed on a Af cm column of EEAE-cellulose equilibrated with starting buffer.

After starting his writing career by writing for " true confession " style pulp magazines like True Story, van Vogt decided to switch to writing something he enjoyed, science fiction.

After leaving school at 17, Ayckbourn's career took several temporary jobs in various places before starting a temporary job at the Scarborough Library Theatre, where he was introduced to the artistic director, Stephen Joseph.

After the tumultuous and disappointing 2007 season, the Falcons made a number of moves, hiring a new General Manager and head coach, drafting a new starting quarterback, and signing a starting running back.

After rallying his troops near Schwennenbach – well beyond their starting point – Eugene prepared to launch a second attack, led by the second-line squadrons under the Duke of Württemberg-Teck.

After starting the 1954 campaign with a two-game split against the Tigers in Detroit, the Orioles returned to Baltimore on April 15 to a welcoming parade that wound through the streets of downtown, with an estimated 350, 000 spectators lining the route.

After the victory Boston's owner sold its star pitcher, Babe Ruth, to the New York Yankees to help funding of a Broadway play, and thus starting a tale of futility which would last 86 years, known as Curse of the Bambino.

After starting the season 15 – 35, though, the team had some success later in the year, going a respectable 30 – 28 in August and September as the youngsters became more experienced.

After defeating, but not subjugating, the Kingdom of Scotland, he declared himself rightful heir to the French throne in 1338, starting what would be known as the Hundred Years ' War.

After passing his examinations in 1752, he soon progressed through the merchant navy ranks, starting with his promotion in that year to mate aboard the collier brig Friendship.

After various Korean border conflicts, war broke out with U. S .- allied South Korea in 1950, starting the Korean War.

After cutting out the hatchlings may lie in their eggshells for hours before starting to dig out of the nest.

After being sent on a lengthy tour of Europe starting 1927 – including London, Paris and towns in Germany – during which he demonstrated his invention to full audiences, Theremin found his way to the United States, arriving on 30 December 1927 with his first wife Katia.

After the 1997 death of Scott Krueger, a new member at the Phi Gamma Delta fraternity, MIT required all freshmen to live in the dormitory system starting in 2002.

After starting a collaboration on heart applications with Robert Weiss at Johns Hopkins, Bottomley returned to the university in 1994, as Russell Morgan Professor and Director of the MR Research Division.

After the introduction of the motel, auto camps continued in popularity through the Depression years and after World War II, their popularity finally starting to diminish with the construction of freeways and changes in consumer demands.

After starting 3 – 7 in 1994, the Giants won their final six games to finish 9 – 7 but missed the playoffs.

After starting 5 – 0 in the 2009 season, New York lost to the likewise undefeated New Orleans Saints at the Superdome 48 – 27, beginning a four game losing streak, in which they lost to the Arizona Cardinals 24 – 17, the San Diego Chargers 21 – 20 and the Philadelphia Eagles 40 – 17.

After La Russa retired as manager of the Cardinals in 2011, Smith became active in the organization again, starting with his stint as a special instructor for the team's 2012 spring training camp.

After everyone has made their decision, the replacement cards are dealt starting at the dealer's left as usual.

After reaggravating a hamstring injury during Game 3 of the 2007 ALDS, Joe Torre removed Roger Clemens from the team's starting rotation.

After starting the engine with a known setting that allowed it to idle, the air valve was opened until maximum engine speed was obtained.

After a while, Prokofiev's mother felt that the isolation in Sontsovka was restricting his further musical development, yet his parents hesitated over starting their son on a musical career at such an early age.

After the Welsh victory at the battle of Llwchwr in January 1136 and the successful ambush of Richard Fitz Gilbert de Clare in April, south Wales rose in rebellion, starting in east Glamorgan and rapidly spreading across the rest of south Wales during 1137.

After the war, Penny had wanted Keith at least to visit her home with her.

After I paid Monsieur Prieur for Dandy, I brought him home, but he was ill at ease and ran away the same night.

After that, it requires several minutes of concentrated work, including six separate and deliberate actions by a minimum of three men sitting at three separate stations in a bomber, each with another man beside him to help, for an armed bomb to be released.

After he had spent the first three years in New York as associate conductor, at Toscanini's invitation, of the NBC Orchestra, he made numerous guest appearances throughout the United States and Latin America.

After a sort of death march during four days without food, Helion and his comrades were shipped by cattle-car to a labor camp at an estate farm in East Germany.

( After graduation from the University of Chicago, Paula taught for two years in the normal school at Valley City, North Dakota, then two years at Princeton ( Illinois ) Township High School.

After complimenting Morgan and the riflemen and saying he was praising them to Congress, too, the ardent Frenchman added he felt that Congress should make some financial restitution to the widow and family of Morris, but that he knew Morgan realized how long such action usually required, if it was done at all.

After baptism Jesus came up out of the water at once, and at that moment heaven opened ; ;

After a tortuous drive in an open truck and a World War 2, army jeep down soggy trails, the band arrived at a small clearing squeezed between a long, low ridge and a creek-filled gully.

After trimming off the excess on the frames and transom which was used to fasten them to the jig at a working height, the top of the side planking is installed.

After more years of concentrated effort, Roy and his brother Max finally established a thriving family business at the old stand.

After drying the sections under the fan, fixing in acetone, and drying at 37-degrees as in the indirect method, the sections were treated with conjugated Af or Af ( undiluted unless mentioned otherwise ) for 5 - 30 minutes.

After `` a prolonged study of the Oder line on a map '', at Teheran, Churchill `` liked the picture ''.

After a conversation with another man, he was able to recount practically everything that had been said but could not describe at all what the other man looked like.

After being located for some years in the Village at the Equinox Pharmacy under the supervision of Mrs. Harry Mercier, it is presently located in the Hill and Dale Shop, Manchester Center.

After 1895 the number increases, and in the next thirty years there is only one year for which there is no dated poem -- 1903, when Hardy was at work on The Dynasts.

After a week of precarious uphill landings and downwind takeoffs, Fogg one day looked down at the shattered yellow wreckage of an Army plane strewn across snow-covered Towne field.

`` After your child's baby teeth are all in -- usually at the age of two and one half to three -- it's time for that first dental appointment '', Dr. Brodie advises.

Dr. Karlis Osis, Director of Research at the Parapsychology Foundation, described the basis for the experiment in a tomorrow article, ( `` New Research On Survival After Death '', Spring 1958 ).

After a flood of protests, they were reinstated at the beginning of 1953.

After finding that its coasts led nowhere, however, he turned north again, toward the main, ice-filled passageway -- and the crew, at first uneasy, then frightened, rebelled.

After an earthquake in Japan in 1793 people on the coast at Tugaru were so terrified by the extraordinary ebbing of the sea that they scurried to higher ground.

After promoting Frost's appearance at the Inauguration, he persuaded the poet to return several months later to give a reading to a select audience of Cabinet members, members of Congress, and other Washington notables gathered in the State Department auditorium.